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The same steps were also followed using a more diluted catalase solution (0.0033 mg/mL or 6.6716.67 units/mL)

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coli Rosetta2DE3pLysS cells transformed with recombinant plasmids were grown to an A 600 of 1.0 at 30 C and the protein induction was carried out at 18 C for 12 h using 1 mM isopropyl--D-thiogalactoside (IPTG) (MP Biomedicals, 11IPTG0001)