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Indeed, it can considerably modify the NP shape, size, surface charge distribution, and susceptibility to aggregation

To address the reactivities of the redox-sensitive cysteinyl residues, we also analyzed the recombinant monothiol roGFP2 variants roGFP2 C151S and roGFP2 C208S and detected small but reproducible glutathionylation-dependent changes of fluorescence at both excitation wavelengths for roGFP2 C151S (SSG) and roGFP2 C208S (SSG) (Fig

Energy levels stabilize after the initial adjustment period

Wang P, Chen D, An JX, Lin SX, Liu T, Li Y, Chen L, He B (2022) Development of a single-step fluorogenic sirtuin assay and its applications for high-throughput screening