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All subsequent manipulations were performed on a Leica Bond Automated Immunostainer

Xiao J, You Y, Chen X, Tang Y, Chen Y, Liu Q, et al

The immunoblot results revealed that CSD significantly depressed the phosphorylated Nrf2 (p-Nrf2) activation, and subsequently reduced the downstream anti-oxidative enzymes activities (i.e., SOD1 and GPx) (Figures 3A,C)

It is hypothesized that transnasal absorption into the bloodstream may be an important way for APP inhalation to act on the ischemic brain injury area