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Streptavidin beads were washed with 50 mM NaAc (pH = 4.5), 50 mM NaAc containing 2 M NaCl (pH = 4.5), and deionized water twice each with vortexing and/or rotation to remove non-specific binding substances, then resuspended in 25 mM ammonium bicarbonate, transferred to glass tubes (VWR), and irradiated with 365 nm UV light (Entela, Upland, CA) for 2 h at RT with magnetic stirring

Shrivastava R, Upreti RK, Seth PK, Chaturvedi UC (2002) Effects of chromium on the immune system

10.1016/s1097-2765(01)00438-5 110 HasegawaY.ShimizuT.TakahashiN.OkadaY

It works by gently modulating the biological pathways of pigment production