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Polyphyllin VII treatment induced apoptosis and inhibited proliferation, migration, tumor spheroid formation, and stemness (induced a reduction in the expression of the stemness markers OCT4 and CD133) in Huh7 and HepG2 cells

255 In contrast to the hepatoprotective effects of CYP1A1 and CYP3A4, the CYP4 family member CYP4A14 is upregulated in fatty livers and significantly exacerbates HFD- and MCD-induced liver lipid accumulation and NASH progression by inducing FAT/CD36 expression in the HFD- and MCD-triggered mouse NASH model

The column was washed with lysis buffer (5 mM imodazole in lysis buffer) and increasing concentrations of NaCl (0.5 mM and 1 mM) followed by increasing concentrations of imidazole (5 mM and 20 mM, in lysis buffer) to remove contaminating proteins