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Targeted delivery of proteins across the bloodbrain barrier

In pulmonary fibrosis studies using bleomycin-induced injury in mice, doses of 2.6-260 g/mL/day administered every other day from day 4-21 reduced inflammatory cell infiltration, decreased interstitial thickness, and reduced TNF- and IL-6 expression

To overcome this problem, a Gly-rich linker was inserted between sequences encoding GSH1 or GSH2 and 3XFLAG tag to generate the pF1-GSH1-lin-3XFLAG and pF1-GSH2-lin-3XFLAG constructs