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Match sampling and normalization to the question (per mg protein, per cell, per tissue mass or surface, account for m when using mito-probes), control for probe loading/photobleaching/pH sensitivity and verify that treatments do not alter probe uptake or peroxidase activity (Krishnamurthy et al

Assessment of the Withdrawal Threshold for the Hind Paw Mice in the four groups were tested for pain sensitivity using von Frey monofilaments (20PC Aesthe, Muromachi Kikai Ltd., Tokyo, Japan) and a hot plate (Variomag, Langenselbold, Germany)