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Briefly, single-indexed DNA libraries were generated from 0.51.5 ng of DNA samples using the NEBNext Ultra II DNA Library Prep kit for Illumina (New England Biolabs)

MT-1 (Melanotan 1, also called afamelanotide) is a synthetic 13-amino-acid analog of alpha-melanocyte-stimulating hormone (alpha-MSH) that selectively activates melanocortin-1 (MC1R) and to a lesser extent MC4R receptors