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After glutathione neutralizes a free radical, the body can recycle it back into its active form and put it back to work

Endocrine 47:146151 Sofic E, Rustembegovic A, Kroyer G et al (2002) Serum antioxidant capacity in neurological, psychiatric, renal diseases and cardiomyopathy

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Since the membranes have a high affinity for proteins, the remaining binding sites of the surface need to be blocked after the transfer to prevent unspecific binding of the detection antibodies