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But it has undeniably shortened its effective lifespan and introduced a variable you can't control

Acta 450, 8389

The filtered supernatant was run over a protein A/G column (Pierce), followed by extensive washing with PBS and the antibodies were eluted with 18 mL 0.1 M glycine pH 2.5 directly captured in 2 mL neutralization buffer (1 M TRIS pH 8.7)