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Humane endpoints were predefined, and any animals showing signs of severe distress or irreversible suffering were promptly euthanized to ensure animal welfare

These markers serve two purposes: they establish your baseline metabolic health and they provide reference points for monitoring changes during the protocol

In Vitro Studies Variables commonly adjusted: Concentration Exposure duration Cell type Endpoint measurements Preclinical Studies Variables commonly adjusted: Dose Delivery route Study duration Experimental objective Appropriate controls should be used to isolate compound-specific effects

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