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Early expression of matrix metalloproteinase-9 (MMP-9) and VEGF after ischemia/reperfusion is associated with microvessels, causing degradation of the vascular matrix and blood-brain barrier leakage (Zhao et al., 2006)

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Stable covalent bond with minimal ligand leakage High binding capacity, very low nonspecific binding Scalable Easily adjusts for sample size and automation Reproducible results Investigating protein structure and function Preparing recombinant proteins for X-ray crystallography Ideal for study of protein interactions with protein or DNA Immunization to raise antibodies against a protein of interest Effective screening protein expression even with crude cell lysates Microscale purification of GST-tagged proteins Glutathione-S-transferase (GST) is a highly soluble and stable 26 kDa enzyme that catalyzes the protective mechanisms of Glutathione (GSH)

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