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This is because mass spectrometry-based proteomics workflows, the preferred method for studying PTMs on a proteome-wide scale, include the reduction and alkylation of disulfide bonds as one of the first steps in sample preparation, which results in the loss of any cysteine PTMs that were present 2

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Cell 84 : 299308 Inoue T, Saito H, Fukushima R, Inaba T, Lin M-T, Fukatsu K, Muto T (1995) Growth hormone and insulin-like growth factor I enhance host defense in a murine sepsis model

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