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10.3390/cancers14246203 10 AyusoJ

In humans, dietary l-carnitine is converted into the atherosclerosis- and thrombosis-promoting metabolite TMAO via 2 sequential gut microbiotadependent transformations: (a) initial rapid generation of the atherogenic intermediate BB, followed by (b) transformation into TMA via low-abundance microbiota in omnivores, and to a markedly lower extent, in vegans/vegetarians

We are constantly working on keeping them as accurate as possible

The resulting construct was biolistically transformed into the WT strain as described previously 54 , and positive transformants were selected on hygromycin (200 g ml -1 ) and confirmed via PCR and Southern blot analysis