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Description

Maintenance phase (post week 8)

Additionally, negatively charged PSS effectively adsorbs positively charged VEGF [342]

This is generally done over 5 weeks, although it may need to be renewed once depending on your metabolism

Supernatant containing the protein portion was added with sample buffer (0.125 M Tris [pH 6.8], 10% [v/v] glycerol, 0.02% SDS, and 1 mM dithiothreitol [DTT]) and subsequently heated at 95 C for 5 min