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Harmonic restraints of 50.0 kcal mol 1 2 were applied to the solutes, including the crystallographic ions, to allow solvent relaxation while preserving the maximum fidelity to the docking pose, and the Andersen temperature coupling scheme 81,82 was used to control and equalize the temperature

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Imerslund-Grsbeck syndrome is an inherited vitamin B 12 malabsorption syndrome that causes megaloblastic anemia and neurologic disorders of variable severity in affected subjects