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They should also evaluate whether pile driving is practical for the site, whether vibration or noise restrictions apply, and whether drilled construction would reduce or increase overall risk

collagenase solution DS with 0.1% (wt/vol) type 2 collagenase and 96 g/mL soybean trypsin inhibitor the proximal tubule suspension was centrifuged for 5 min at 170 g, washed, and then resuspended into the appropriate amount of culture medium (1:1 DMEM/F12 without phenol red and supplemented with heat-inactivated 1% FCS, 15 mmol/L Hepes, 2 mmol/L L-glutamine, 50 nmol/L hydrocortisone, 5 g/mL insulin, 5 g/mL transferrin, 5 ng/mL sodium selenite, 0.55 mmol/L sodium pyruvate, 10 mL/L 100 nonessential amino acids, 100 IU/mL penicillin, and 100 g/mL streptomycin buffered to pH 7.4 (osmolality of 325 mOsmol/kg H 2 O)

Cultures may be difficult as these organisms are fastidious

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