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One of the limitations of our study is that we measured expression levels on transcriptional level only and the activity and quantity of the studied enzymes was not evaluated.

Identification of a novel function of hepatic long-chain acyl-CoA synthetase-1 (ACSL1) in bile acid synthesis and its regulation by bile acid-activated farnesoid X receptor

After incubation on ice for 1 h, the homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays

These are not FDA-registered manufacturers in the same sense as Pfizer or Fresenius Kabi they operate under pharmacy compounding regulations rather than drug manufacturing regulations