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2a for the detailed strategy)

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In practice, stacking multiplies uncertainty: more compounds more chances of impurity/contamination more pathways affected more interaction risk more variables harder to know what helped (or harmed) If you take only one clinical principle from this article: the more experimental the stack, the more conservative your risk posture should be

Theoretical and experimental investigation of thermodynamics and kinetics of thiol-michael addition reactions: a case study of reversible fluorescent probes for glutathione imaging in single cells