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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work

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5-Amino-1MQ Vials (50 mg each): 1 week (5 mg/day) 1 vial 2 weeks (5 mg/day) 1 vial 4 weeks (5 mg/day) 2 vials Insulin Syringes (U-100): Per week (once daily): 7 syringes Per week (BID): 14 syringes 4 weeks (once daily): 28 syringes Bacteriostatic Water (10 mL bottles): Use 4.0 mL per vial for reconstitution

Potassium replacement therapy should be administered if necessary