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Global cerebral ischemia from cardiac arrest during the light (inactive) phase impaired survival and exacerbated outcome characterized by increased microglial activation, degenerating neurons, and proinflammatory cytokines in the hippocampus compared to dark (active) phase in C3H mice (Weil et al., 2009)

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The 2^-Ct method was used to calculate fold changes, with VC_CTL as the reference sample for both qRT-PCR and transcriptome data

Other patients may be high-risk surgical candidates or have contraindications to surgery