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The short half-life and rapid excretion of butyrate in the human body limit its therapeutic effect, thereby restricting its practical application [96, 97]

They cannot supply the entire organic requirement, thus recommending the use of food supplements as the primary source of nutrients (Shay et al., 2009)

Separation was carried out using a 10 cm long C18 UPLC column with an ammonium acetate buffer (A) and methanol (B) as the mobile phase for gradient elution (575 % B in 10 min), at 40 C and 020 ml/min