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100 YakabiK.NoguchiM.OhnoS.RoS.OnouchiT.OchiaiM.et al (2011)

Boulton, B., Singleton, V.L., Bisson, L.F

For all methods, following thiol extraction with the appropriate solvent, cells were incubated on a shaker for 1 h at 4 C and then centrifuged at 20,000 g to remove insoluble material