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Following digestion of the pTAT-HA vector and TrxR1 insert, T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) was used to ligate TrxR1 into the Nco I and Eco RI sites in the similarly digested pTAT-HA vector

During GBM development, various aberrations in epigenetic modifications, including DNA methylation, histone acetylation, and N6-methyladenosine (m6A) RNA modification, occur [16]