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RNA extraction and quantitative real-time polymerase chain reaction (RT-qPCR) Tissues from challenged mice were harvested and placed in 1 mL TRIzol Reagent in 2 mL RNase/DNase-free Omni tubes (Omni International), which contained beads for homogenization

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Only after 48 h, sugar accumulation was observed reaching a maximum of 4.5 g L 1 for LmgshF , followed by 3.9 g L 1 for GSH1 and the reference strain