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This is why microdosing protocols almost always require the 10mg/ml concentration, where the volumes are large enough to measure reliably

Allergy 73 Suppl 104 , 523 (2018)

First-line gefitinib in patients with advanced non-small-cell lung cancer harboring somatic EGFR mutations

In the beginning of HPLC-MS/MS analysis of mycotoxin biomarkers, these criteria were not defined and a lot of the ground-breaking research on AFB1 exposure using AFB1-Lys as biomarker was probably only possible because the single MRM transition m / z 457.3394.2 was regarded as sufficient for identification (McCoy et al