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doi: 10.1016/j.autrev.2020.102688 174 HansenALBuchanGJRhlMMukaiKSalvatoreSROgawaEet al

LIAS binds two [4Fe-4S] clustersthe first mediates a radical SAM reaction that activates the aliphatic chain for sulfur attachment, while the second acts as a sulfur donor and must be replenished after each reaction 58

This allows researchers to observe the spatial distribution of histone modifications in the cell, such as in different types of chromatin or different cellular structures