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Females are about five to eight times more likely than males to have a thyroid condition

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from 100 M stock in DMSO) in ERB buffer for 30 min at 37 C in ambient CO 2

For miRNA expression analysis, 200 ng total RNA was taken for polyA tail addition followed by cDNA synthesis using Quanta Biosciences 95107-025 qScript miRNA cDNA Synthesis Kit according to the manufacturers instructions (Mestdagh et al, 2014)