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Determination of Nitric Oxide (NO) Production The nitrite (NO 2 ) was estimated colorimetrically at 12 and 24 h post CLP on the basis of reduction of nitrate to nitrite using Griess method ( Bacterial Load Determination For determination of colony-forming units (CFU) in blood and peritoneal fluid of mice ( n = 6 per/group), 10 L of samples were diluted with sterile Phosphate-Buffered Saline (PBS) 1:10, and then 10 L of each dilution was cultured in Mller Hinton Agar and incubated at 37 C for 24 h

All animal experiments were approved by the Committee on Animal Research at Tohoku University, and performed in agreement with the the Association for Research in Vision and Ophthalmology (ARVO) statement for the use of animals in ophthalmic and vision research and ARRIVE (Animals in Research: Reporting In Vivo Experiments) guidelines