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shRNA sequences used are as follows: Piezo1 shRNA: 1-AAGTACGACCTGGTGCAAC, 2 TCACGGGCATCTACGTCAA, 3 TGCTGTGCCTCACGTGTT

[48] Analysis results of racemic ethyl 4-chloro-3-hydroxybutyrate are as follows: [49] 1 H-NMR (CDCl 3 ) = 1.28 (3H,t), 2.62 (2H,d), 3.53 (lH,br), [50] 3.60 (2H,d), 4.20 (2H,q), 4.33 (lH,m) [51] [52] Example 3: Preparation of ethyl (RV4-chloro-3-hvdroxybutyrate (4) [53] 40 g (10% w/v) of racemic ethyl 4-chloro-3-hydroxybutyrate was added to a flask containing 360 mL of 0.1M potassium phosphate buffer (pH 8.0) to which 4 g (1% w/ v) of immobilized lipase Novozyme 435 was then added, followed by reacting at 30 0 C

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Wang H, Bell M, Sreenivasan U, Sreenevasan U, Hu H, Liu J, Dalen K, Londos C, Yamaguchi T, Rizzo MA, et al