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10.1161/ATVBAHA.117.309249 59 FerranteA.NandoskarM.WalzA.GohD

The compounds used were IGF-1 alone (100 nM, Genentech, San Francisco, CA, USA), cGP alone (100 nM, Bachem AG, Switzerland), IGF-1 + cGP (100 nM), GPE alone (100 nM, Sigma), GPE + IGF-1 (100 nM) and IGF-1 + cGP + GPE (100 nM)

Meanwhile, the activity of nicotinamide adenine dinucleotide (NAD + )-dependent deacetylases Sirtuins [especially sirtuin 1 (SIRT1) and sirtuin 3 (SIRT3)] decreases due to NAD + depletion, leading to hyperacetylation of multiple target proteins, which tends to enhance NF-B signaling and inhibit fatty acid oxidation (52, 53)