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Cell culture and lysates Tumorigenic cell lines LNCaP (CRL-1704), DU-145 (HTB-81), and PC-3 (CRL-1435) and the non-tumorigenic cell line RWPE-1 (CRL-11609), and COS-7 cells (CRL-1651) were purchased from American Type Culture Collection (ATCC, Manassas, VA), cultured according to ATCCs instructions and supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin