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Correlation analysis between immune cells and differentially expressed complement proteins revealed that B cells negatively correlated with C8A, C8B, C8G, and F11, while Tfh cells positively correlated with C8G and MASP1 ( Figure 6B )

Morphometrical data were obtained as previously described ( Total RNA Isolation, cDNA Synthesis, and Relative Real-Time PCR RNA isolation, cDNA synthesis, and relative real-time PCR were performed as previously described ( GAPC1 ) was used as a constitutively expressed reference gene (Supplementary Table 1) were designed using VectorNTI Advanced 10 software (Thermo Fisher Scientific)

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Different individuals use different words to describe the feeling of breathlessness, which is also influenced by other factors such as mood