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The supernatant was collected and concentrated using a Microcon 3,000 molecular weight cutoff filter (Millipore, Bedford, MA, USA) following three washes with 300 l of 50 mM Tris buffer

Dry red wine seems to have the least amount of negative health effects due to its higher polyphenol content and beneficial bacteria, she says

Briefly, GMSCs were cultured in the complete culture medium: -minimum essential medium (-MEM: Invitrogen) supplemented with 10% fetal bovine serum (FBS: Zen-Bio, Inc., Durham, NC), 1% antibiotics (100U/ml penicillin/100 g/ml streptomycin