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Homogenates were centrifuged (Eppendorf 5424) at 4,000 g for 10 min at 4 C, and the supernatants were aliquoted into pre-labelled tubes for immediate assay or frozen storage at -80 C until processing

These limitations collectively lead to transient and unpredictable therapeutic effects, underscoring the need for mechanistic clarification and technological innovation before mitochondrial transplantation can advance toward clinical application

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Their combined structural selectivity supports advanced investigation into tissue-interaction mapping within controlled research environments