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In vitro kinase assay and in vitro IC 50 determination The Phosphorylation-Assisted Luciferase Complementation assay (PhALC) was developed to measure JNK activity in vitro for fast and cost-effective identification/characterization of any compound blocking MAPK activity 35

For example, in the cancer cells, higher temperature can decrease the expression of P-glycoprotein (P-gp) and multidrug resistance-associated protein 1 (MRP1) associated with drug resistance 236

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Consistent with this conclusion, the Aspergillus oryzae GUS has been shown to more efficiently catalyze the formation of glycyrrhetic acid monoglucuronide from 18 when the loop 3 and loop 5 residues, corresponding to E452 and V370 in Rg GUS, respectively, were replaced with polar residues, such as histidine and glutamine 34