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doi:10.1016/j.yfrne.2007.10.005 pmid:18093638 Fukata Y, Fukata M (2010) Protein palmitoylation in neuronal development and synaptic plasticity

The preparation of a 50% iodixanol working solution (Solution D) ensures that the concentrations of EDTA (and MgCl, if included) and buffer are constant in all gradient solutions