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The conservative approach: if the vial was at room temperature for more than 24 hours, assume partial degradation and adjust experimental doses accordingly or source fresh material

The experiments with isolated mitochondria (0.5 mg/mL) were carried out at 28C with a standard reaction medium containing 15 M Ca 2+ , 10 mM Hepes, 125 mM sucrose, 2 mM K 2 HPO 4 , 65 mM KCl, 1 mM MgCl 2 , and 5 mM glutamate plus 5 mM malate (pH 7.2)

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