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To PCR-amplify the specific cleavage product, a homopolymetic tail was added to the 3-end of the cDNA, and two rounds of PCR amplification were performed using NGF GSP1 (5-CCATGCAGTCCTTATAATTTAAAATAATTTAC-3) and nested with PCR with NGF GSP2 (5-AATTTACAGGTTGAGGTAGGGAGG-3), which allowed amplification of unknown sequences between the GSP2 and the 5-end of the mRNA

Identifies side reactions or degradation products