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Retrieved from Van Sebille, Y.Z., Stansborough, R., Wardill, H.R., Bateman, E., Gibson, R.J., & Keefe, D.M

DCA, a key mediator of hostile bile effects, directly impairs CD8 + T cell function by dysregulating plasma membrane Ca + ATPase (PMCA)-mediated calcium efflux, disrupting intracellular Ca + homeostasis and blunting nuclear factor of activated T cells (NFAT)2 signalingcritical for effector cytokine productionthereby accelerating CRC progression (239, 240)

Collagen targeting peptide (T ECM ) was prepared by automated solid-phase peptide synthesis at a 100 mol scale on a rink amide resin using standard Fmoc-based protocols 30 (Supplementary Fig

Approximately 10 ng of RNA was used for RT-qPCR assays using the Luna Universal One-Step RT-qPCR kit (New England Biolabs)