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Furthermore, the higher level of intracellular ROS may damage the mitochondrial membrane and the electron-transport chain, leading to the more ROS and amplifying the oxidative imbalance, also referred to as ROS-induced ROS release (Zorov et al., 2014

Researchers designing combination protocols typically run controls with each compound in isolation alongside the combination arms to distinguish additive from synergistic effects and to identify which compound is driving each observed outcome