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47 , D506D515 (2019)

Tracheotomy was performed and the animals were allowed to breathe spontaneously through the tracheal cannula

15, mjad071

Briefly, sera or peritoneal fluids (50l) were treated with 10% metaphosphoric acid to precipitate protein (30min, 4C) and then centrifuged for 15min at 18000g at 4C