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Chem Biol Interact 5(387) Suzuki T, Yamamoto M (2015) Molecular basis of the Keap1-Nrf2 system

After determining the linear range of the reaction over time, pkat values of each protein in the presence of 1 mM -EC, 2.5 mM ATP and 100 mM glycine were measured in triplicate, and compared to a standard curve of free phosphate