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More recently Yonezawa et al

Cell (2011) 144:81023

GB112099, Servicebio, Wuhan, China) and PNP (1:300

Step 2 Membrane Binding and Insertion: Upon binding to membrane-localised HDM-2, PNC-27 inserts into the lipid bilayer via its C-terminal transmembrane-penetrating domain, anchoring the PNC-27HDM-2 complex within the cell membrane