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A mouse model of hepatocellular carcinoma: Ectopic expression of fibroblast growth factor 19 in skeletal muscle of transgenic mice

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For immunohistochemistry, after dewaxing and hydration, the sections were incubated with 3% hydrogen peroxide-methanol solution at room temperature for 15 min to quench endogenous peroxidase activity and 1 mmol/l levamisole for 20 min to inhibit endogenous alkaline phosphatase activity