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The RNAprotein complexes were captured on Dynabead protein A (Thermo Fisher, 10006D) at 4 C for 90 min, washed with NT2 buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM MgCl 2 and 0.05% NP-40) and then incubated in NT2 buffer containing DNase I (Qiagen, 79254) at 37 C for 10 min

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Many cases of idiopathic male infertility show elevated oxidative markers in semen, indicating that oxidative stress is a frequent, underrecognized cause

Such pathways are also involved in HC loss due to non-traumatic causes