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Association between vitamin D deficiency and mortality in critically ill adult patients: A meta-analysis of cohort studies

Media was replaced 24 h later and confluent cells were treated with 2 l of supernatant harvested from BMDM experiments

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Sections were then briefly immersed in wash buffer and incubated with a mixture of guinea pig anti-insulin and mouse anti-glucagon (both at 1:600 dilution in antibody diluent from Cell Signaling) and incubated for 90 min at 37 C