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Direct and potent regulation of gamma-secretase by its lipid microenvironment

Anesthesia and euthanasia protocol Each rat was anesthetized using 23% isoflurane in oxygen

Hematin (20 M) was dissolved in varying concentrations of GSH (05 mM) and the difference in absorption between 618 nm and 655 nm was plotted against GSH concentration, leading to the determination of the affinity constant, Ka, 5.0 10 4 M 1

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