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Six different injection techniques for CT injection were performed on each specimen, including palmaris longus, ulnar to flexor carpi radialis, trans-flexor carpi radialis, volar radial, volar ulnar, and direct through the palm techniques

This variation is grouped into two main classes, the shorter class I and the longer class III

When administered as an injection, B12 is absorbed 100% into the body, whereas when taken orally, only 40% is absorbed due to the livers filtration functions

For isozymic detection, the partially purified protein was loaded in 12% non-denaturing gel and electrophoresed in 10 mM TrisHCl (pH 6.8) under 1 V/lane against the tracking dye as bromophenol blue