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Immunofluorescence labeling of HSD3B in rat testes and Leydig cells Paraformaldehyde (PFA, 4%)-fixed testis sections or isolated Leydig cells (LCs) were subjected to immunofluorescence staining of 3-hydroxysteroid dehydrogenase (HSD3B)

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For example, inhibitors targeting histone demethylases (such as KDM5 inhibitors like CPI-455 or ryuvidine) have demonstrated efficacy in preclinical models by preventing the reversible repression of chromatin states necessary for DTP survival ( 4.2 Exploiting metabolic vulnerabilities of DTP cells Metabolic reprogramming observed in DTP cells provides another therapeutic opportunity